Microfluidics for Rapid Detection of Live Pathogens

Rapid, sensitive, and selective detection of live pathogens remains a key priority for quality control risk assessment. While conventional methods often require complicated workflows, costly reagents, lab equipment, are time-consuming, rendering them inadequate field testing low-resource settings. Increased attention has been drawn to developing alternative low-cost rapid detect on-site in different environmental matrices. Among them, microfluidic devices that integrate various laboratory functions miniaturized manner have proven be promising tool the sensitive pathogens. Herein, development specifically designed is discussed along concise summary novel microfluidics systems recently developed, contrasted regarding assay time, limit detection, target organisms. These include variety micro total analysis (µTAS) paper-based analytical (µPADs) combination with molecular traditional cell techniques, such as culture, DNA intercalating dyes, resazurin, immobilized bioreceptors (e.g., aptamers capture antibodies). Furthermore, insights on future perspectives pathogen highlight method provided.